GENOME DE NOVO SEQUENCING

For a genome that is to be sequenced for the first time, we offer our De Novo Sequencing Service on the Illumina platform.

BaseClear's De Novo sequencing service:

• We offer single read, paired-end and mate-pair sequencing on the Illumina  platform
• The final sequencing strategy is determined in close collaboration between the customer and our product specialist 
• Starting material is a minimum of 10 ug genomic DNA (minimal concentration 200 ng/uL) 
• Multiplex options available to sequence up to 24 samples in a single lane by adding sequence indexes to a library 
• Data is supplied as raw sequencing data (FASTQf) 
• Optional bioinformatics and gap closure possibilities
• Delivery time is dependent on when we receive the samples, run type and number of lanes, please discuss with our product specialist

Optional: Bioinformatics

• De novo assembly: to assemble the sequencing reads into a number of contig or scaffold sequences.
• Downstream options such as annotation, genome screening and genome analysis can also be performed.
• The bioinformatics strategy will be determined in close collaboration between the customer and our bioinformatics specialist

Optional: Gap closure possibilities

• We can offer gap closure options based on a combination of direct Sanger sequencing, Next Generation Sequencing and long range PCR-based approaches