GENOME-WIDE METHYLATION ANALYSIS
The EpiQuest™ DNA Methylation Analysis Platform combines BaseClear's Next-Gen sequencing with Zymo's well-established bisulfite technologies for the most comprehensive DNA methylation analysis service available. Streamlined sample prep, cutting-edge bioinformatics, and consolidated data delivery take the difficulty out of methylation characterization and simplify epigenetic biomarker discovery.
- Single Base Resolution DNA Methylation Analysis
- Next-Gen bisulfite sequencing platforms for DNA methylation analysis
- Streamlined workflows with comprehensive bioinformatic analysis
- High quality publishable data delivery
- Low DNA input (<500ng genomic DNA)
- Applicable to a broad range of sample sources (human, mouse, rat, plant, etc.)
- Customizable, rapid turnaround at an affordable cost
How does the EpiQuest™ Genomic Service work?
EpiQuest™ Genome-Wide Service expands upon the conventional RRBS (Reduced-Representation Bisulfite Sequencing) method to greatly increase sequence coverage. Genomic DNA is first digested by restriction endonuclease enzymes to produce fragments rich in CpG sites. These fragments are recovered, subject to bisulfite conversion, and sequenced to give single nucleotide resolution sequencing data. This provides a statistically significant representation of CpG dinucleotides throughout the genome. Through the utilization of a multiple enzyme digestion and an optimized workflow (see below), the EpiQuest™ team at Zymo Research has been able to expand coverage to ≥ 75% of gene promoters and ≥80% of CpG islands (for human samples).
The EpiQuest™ Whole-Genome Service utilizes a streamlined library preparation and sophisticated alignment algorithms to deliver Whole Genome Bisulfite-Sequencing that covers > 80% of all the bases in the human genome at single-base resolution.
Full Service
The Full Service package removes all complication from methylome sequencing - simply submit DNA samples and receive high-quality data from our comprehensive bioinformatic analysis. This package is recommended for customers without an extensive bioinformatics background.
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Basic Service
The Basic Service Package will provide Next-Gen sequencing data aligned to the reference genome using our proprietary pipeline and three UCSC genome browser tracks. This option is recommended for users well-versed in bioinformatics.
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Customizable Service
EpiQuest™ service can be adapted to fit the needs of the investigator. There are many options for difficult samples, extra bioinformatic analysis, locus-specific validations, different organisms, and more.
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Why BaseClear?
Outsourcing of your DNA project to BaseClear offers a number of direct and indirect advantages. Results are quickly available and we offer professional, customer-oriented and customized services.
