FULL SERVICE PACKAGE

The EpiQuest™ DNA Methylation Analysis Platform combines BaseClear's Next-Gen sequencing with Zymo's well-established bisulfite technologies for the most comprehensive DNA methylation analysis service available. Streamlined sample prep, cutting-edge bioinformatics, and consolidated data delivery take the difficulty out of methylation characterization and simplify epigenetic biomarker discovery.

Pricing
For pricing information and a personalized quote, please inquire via our contactform. Discounts are available for volume orders.

Turnaround
Fast results, projects are completed in about 5-6 weeks for most samples.

Services Performed

• Genomic DNA Sample Validation 
• Sample Processing and Library Preparation 
          1. Endonuclease Digestion
          2. End Modification
          3. Adaptor Ligation
          4. Bisulfite Conversion
          5. Limited Amplification 
• Next-Gen Sequencing 
• Sequencing Validation and Bioinformatic Analysis 
• Bioinformatic Pipeline Output and Data Delivery  

Output and Deliverables

• Correlation Data (scatter plot) 
• Sequence Coverage Information (pie chart) 
• Hierarchical Clustering (heat map) 
• Methylation Frequency (barchart) 
• Methylation Index (percentage) 
• Differentially Methylated Gene Identification (table) 
• Genome Browser Tracks
          1. CpG Track
          2. Endonuclease-digested Fragment Track
          3. Next-Gen Sequencing Read Track 
• Aligned Next-Gen sequencing data 
• And more! More than 35 files will be delivered from our comprehensive bioinformatics pipeline!

Basic Service
The Basic Service Package will provide Next-Gen sequencing data aligned to the reference genome using our proprietary pipeline and three UCSC genome browser tracks. This option is recommended for users well-versed in bioinformatics.
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Customizable Service
EpiQuest™ service can be adapted to fit the needs of the investigator. There are many options for difficult samples, extra bioinformatic analysis, locus-specific validations, different organisms, and more.
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