RE-SEQUENCING SERVICE

Our Re-sequencing Service may be helpful to discover and confirm SNPs, to identify chromosomal rearrangements, to map break points and to detect rare variants.

BaseClear's Re-sequencing service in short:

• We offer single read, paired-end and mate-pair sequencing on the Illumina platform
• Our product specialist will help the customer to decide which combination should be performed 
• Starting material is a minimum of 10 ug genomic DNA (minimal concentration 200 ng/uL) 
• Sequence enrichment can be performed for specific regions 
• Multiplex options available to sequence up to 12 samples in a single lane 
• Data is supplied as raw sequencing data (FASTA or scarf) 
• Delivery time is dependent on when we receive the samples, run type and number of lanes, please discuss with our product specialist

Optional: Bioinformatics

• Reference alignment: the customer will receive the contigs that are created during the alignment 
• Downstream options such as SNP/indel identification and discovery 
• The bioinformatics strategy will be determined in close collaboration between the customer and our bioinformatics specialist

Optional: DNA target enrichment

• BaseClear offers the possibility to enrichment for specific regions of the genome, e.g. exome enrichment 
• The enriched samples can then be sequenced giving a high level of coverage for the regions of interest