TRANSCRIPTOME ANALYSIS (RNA-SEQ)

Our mRNA-Seq service allows essentially unlimited discovery and profiling of the entire transcriptome. With no probes or primers to design, the mRNA-Seq method can deliver unbiased and unparalleled information about the transcriptome.

BaseClear's Transcriptome sequencing service:

• The sequencing strategy is determined in close collaboration between the customer and our product specialist 
• Starting material is a minimum of 20 ug total RNA in a maximum of 40 uL (RIN-value of 8.0 or greater) 
• Data is supplied as raw sequencing data (FASTQ) 
• Delivery time is dependent on when we receive the samples, run type and number of lanes, please discuss with our product specialist

Optional bioinformatics

• De novo transcriptome assembly and annotation or alignment against a know reference sequence. In addition normalized gene expression levels will be calculated.
  The customer receives both the alignments and gene expression table.
• Downstream options such as cSNPs, novel transcripts, novel isoforms, alternative splice sites, allele specific expression and rare transcripts 
• The bioinformatics strategy will be determined in close collaboration between the customer and our bioinformatics specialist