96-WELL QUICK SHOT SEQUENCING SERVICE

For routine sequencing analysis of large numbers of samples, BaseClear offers a special high throughput service. Our state-of-the-art robotics guarantee fast and accurate results, and leads to a significant cost reduction compared to single runs.

BaseClear's 96-well Quick Shot sequencing service in short:

• DNA purification and premixing of DNA and primer is done by the customer in 96-well plates 
• Samples can be delivered as either half (up to 47 samples) or full (up to 95 samples) plates
• Sequence reactions are loaded onto our sequencers with a run-time corresponding to 550 or 1000 bases
• Data analysis occurs automatically by PHRED, the international standard software for superior base calling and sequence data interpretation
• No manual data editing 
• Delivery time: 2-4 working days

Purified DNA premixed with primer

• For purified plasmid samples, submit 0,75 µg plasmid DNA mixed with 25 pmol of primer in a total volume of 20 µl 
• For PCR product samples, submit 15 ng per 100 bp PCR product with 25 pmol of primer in a total volume of 20 µl