FULL SEQUENCING SERVICE

Full sequencing means full service and high quality. This option guarantees the best sequencing results when it comes to read length, quality of data and customer wishes.

BaseClear’s full sequencing service in short:

• DNA concentration will be measured (via fluorescence measurement) prior to the sequencing reaction in order to obtain optimal results 
• Single sequencing reaction with a run-time corresponding to either 1000 bp (long run) or 550 bp (short run) 
• Sequencing data are automatically analysed by PHRED, the international standard software for superior base calling and sequence data interpretation 
• Poor sequence runs that do not meet our quality standards are reviewed manually 
• Failed runs are repeated (if necessary under specific conditions) at no additional charge 
• You will receive a full report of the sequencing reaction, including sequence data in FASTA and SCF format 
• Delivery time: 2 working days

Primers

• Standard primer, provided by BaseClear free of charge 
• Custom primer, sent with the order 
• Client-specific primer, which is already in-house 
• Custom primer designed and synthesised by BaseClear (additional costs and one day delivery time)

Optional: DNA Purification

• Customers can choose to submit raw PCR product or bacteria instead of purified PCR product or plasmid DNA 
• BaseClear will purify DNA out of these samples at additional costs and delivery time (1 day)

FREE TRIAL FOR EVALUATION

We offer the possibility to get acquinted with our full sequencing service.

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