GENOTYPING GENERAL

Genetic Variation Analysis
BaseClear offers a broad package of services for the analysis of genetic variations. BaseClear has the technical tools and scientific capabilities to, for example, screen for mutagenesis, single or multiple SNPs and tandem repeat numbers (VNTR or STR).

Genetic variations can affect the development of diseases and the response to pathogens and drugs. The study of such variations is also important in other research fields, such as crop and livestock breeding programs. Genetic variations may be present naturally or have been introduced by genetic modification.

Customised service
We offer various custom genetic variation analysis services, based on a number of techniques. Both low and high throughput screening projects can be conducted, based on standard or newly designed assays. Genotyping can be performed both for known genetic variations and for new, undiscovered mutations. Our interactive project management will ensure that all your ideas are taken into account. In the case of a newly designed assay, we will store the assay so that our customers can easily ship new series of samples for subsequent screening. Full service including DNA purification and primer design. Samples may be delivered as purified DNA or as raw material. BaseClear has fully automated systems to isolate and quantify DNA from various sources in high throughput format. Primers may be provided by the customer or designed and synthesised by BaseClear.

Real-time PCR
Real-time PCR is a fast and accurate method for determining copy numbers of one or more genes in large number of samples. In this method, the amount of amplified DNA is coupled to the measured fluorescence intensity via the presence of a fluorescent reporter molecule. Based on the number of PCR cycles that is needed to pass a certain threshold value, it is possible to calculate the initial amount of RNA or DNA. The technique can also be used for SNP and mutation screening. BaseClear can perform standard real-time PCR assays or develop a customised new assay, including the required set of reference genes.