Custom Projects

Contact

Derek Butler
Call: +31 (0)71 523 3917
Contact us

DNA cloning, library construction and protein expression are essential for many bio-scientific research projects. BaseClear is ready to assist you with this work thus giving you more time and freedom to focus on the core aspects of your research. Our specialists have extensive experience in the design and execution of such projects and we will tailor each project specifically to suite the needs of the customer. Once you have specified the DNA construct, protein or library you would like produced we will work with you to design and execute the project, of course we also guarantee absolute confidentiality.

The custom services provided by BaseClear fall into the following categories:

Custom Cloning

Comprehensive cloning service including selection of the best cloning strategy, isolation or synthesis of the required DNA fragment, cloning and verification of final construct through DNA sequencing. Extensive experience with complex and challenging cloning projects.

Protein Expression and Purification

Cloning of your gene of interest into an appropriate expression vector. Analysis and optimisation of protein expression in E. coli. Purification of the expressed protein. At all stages the expression and purification strategies used will be discussed and agreed with the customer. Our in-house gene synthesis and DNA sequencing services enable us to optimise codon usage and verify a constructs DNA sequence quickly and without any material leaving the company.

Library Construction

Library construction from purified RNA/DNA or raw material.

RNA Synthesis

Synthesis of RNA by in vitro transcription for use in e.g. microinjection experiments or to serve as control RNA source in NASBA or RT-PCR procedures.