| Derek Butler |
|---|
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Call: +31 (0)71 523 3917 |
| Contact us |
The expression level of a protein will be optimized in a number of stages, tailored specifically for each protein and agreed with the customer before initiation. These stages are: |
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BC-209 - GenesynthesisSynthesis of a synthetic DNA fragment |
From gene to protein
BaseClear offers a complete package of services from gene synthesis to protein purification. Our experience in this field coupled with our in-house gene synthesis and DNA sequencing services enable us to complete projects in a fast and flexible manner. Regular and open communication with our customers is an essential aspect of all projects we perform.
Click to enlarge Custom service: protein purification
In case of good protein expression, we
can purify the protein by means of affinity columns.
Depending upon the expression level, up to 30 mg of protein can be produced. Together with the
customer we can optimize the buffer and elution conditions to obtain a protein that has the maximum possible activity.
Results
As a result of the agreed procedure, we will deliver:
- Gene cloned into an expression vector, in an E. coli expression strain.
- Small aliquot of tagged purified protein (provided the expression level is sufficient).
- If protein purification is requested, we can deliver up to 10-20 mg of purified protein.
- Report of the expression analysis and (if performed) the protein purification.
| Standard service: | Custom possibilities: | |
| Gene synthesis | - | De Novo synthesis with optimized codon usage |
| Cloning | Cloning into a standard expression vector | Cloning optimization |
| Expression | Expression in E. coli under standard conditions | Analysis of expression in Bacillus (gram+ host) |
| Expression level | Expression level analysis | Optimization of expression level |
| Protein purification | Basic tag-purification to demonstrate tag-functionality | Optimization of protein purification by affinity tag |
| Delivery time | 2-4 weeks | Indication upon request |
| Price | Cloning € 450 Expression level analysis € 300 Total costs feasibility study € 750 In case of no expression € 600 |
Indication upon request |
BaseClear will only use organisms and DNA that is classified under our GGO-license.
BaseClear guarantees the complete confidentiality of all client information, project data and biological samples during and after
the termination of the contract.
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |