Protein Expression and Purification
| Derek Butler |
|---|
 |
Call: +31 (0)71 523 3917 |
| Contact us |
Cloning and expression of your gene of interest |
Analysis and optimisation of the expression level |
Protein purification |
Interactive project management and open communication |
Cloning of your gene of interest into an appropriate expression vector. Analysis and optimisation of protein expression in E.coli. Purification of the expressed protein. At all stages the expression and purification strategies used will be discussed and agreed with the customer. Our in-house gene synthesis and DNA sequencing services enable us to optimised codon usage and verify a constructs DNA sequence quickly and without any material leaving the company.
Standard servicecloning into standard expression vector followed by sds-page or western analysis to determine expression level in E. coli |
Custom servicecloning into expression vector, analysis of expression level by sds-page or western analysis, optimisation of expression level, |
Protein purificationpurification by affinity TAG (HIS-tag) & delivery of an aliquot of the protein of interest |
Analysis of expression level in Bacillus (gram+ host) |
BC-209 - GenesynthesisSynthesis of a synthetic DNA fragment |
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |