High Throughput Sequencing
| Richard de Winter |
|---|
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Call: +31 (0)71 523 3917 |
| Contact us |
| Order now |
| Whole plates, minus well H12 (95 samples) |
| Samples delivered in MTP format |
| 500 or 750 bp. |
| Delivery time: 3-5 working days |
BC-200B - HT PCR purificationPurification of PCR products in 96 well format prior to downstream procedures. |
BC-200A - HT Plasmid prepPurification of plasmid DNA from 1,5 ml. of E.coli culture in 96 well format. |
BC-207A - HT DNA amplificationPerformance of 95 PCR reactions (with one primercombination) in a 96-well plate on bacterial colonies or plasmid DNA. |
BC-500 - Sequencing Primer design and synthesisDesign of a sequencing primer, including synthesis of one primer on 40 nM scale |
High Throughput Sequencing
Many samples, fast results
Today customers need to do more and more routine sequencing analysis on a large number of samples. BaseClear has the capacity to do this and performs high throughput sequencing in a fully automated manner. We use state-of-the-art robotics to guarantee fast and accurate results.
Samples for high-throughput sequencing must be delivered in 96- or 384-well plates.
1 type of sample can be submitted per plate: you may choose from bacteria (in glycerol or culture, first a HT-PCR/purification will be performed), purified plasmid, purified and raw PCR-products. Please leave well H12 empty for our sequencing control.
The PCR/sequencing reactions can be performed with your own choice of primer: standard primer or custom primer sent with the order or made by us.
Sequence reactions are loaded on our sequencers with a maximum run-time corresponding to 500 or 750 nucleotides.
BC-126S - HT shortSingle sequencing reaction. Data delivered in 24 hours. No editing of data and rerun on request. Short run with readlength of max. 550 bases |
BC-126L - HT longSingle sequencing reaction. Data delivered in 24 hours. No editing of data and rerun on request. Short run with readlength of up to 1000 bases |
BC-127S - HT Quickshot shortsingle sequencing reaction, at least 95 samples delivered in MTP and sequenced with one primer. Short run with readlength of max. 550 bases |
BC-127L - HT Quickshot longsingle sequencing reaction, when at least 95 samples delivered in MTP and sequenced with one primer. Long run with readlength of up to 1000 bases |

