Project Sequencing
| Richard de Winter |
|---|
 |
Call: +31 (0)71 523 3917 |
| Contact us |
| Order now |
| Full Service |
| Complete sequencing of constructs |
| Including DNA purification |
| Including primer design |
| Comparison to reference sequence |
| Delivery time: >1 kb per week |
BC-500 - Sequencing Primer design and synthesisDesign of a sequencing primer, including synthesis of one primer on 40 nM scale |
BC-214 - plasmid library/cosmid,BACconstruction of a plasmid DNA library from a cosmid or BAC |
BC-218 - plasmid library/genomic DNAconstruction of a plasmid DNA library from genomic DNA |
BC-710 - cDNA libraryconstruction of a plasmid DNA library from cDNA |
Project Sequencing
Project Sequencing is BaseClears premier service when it comes to DNA sequencing. All steps necessary to obtain a reliable contig sequence from your clones are included such as DNA isolation from bacteria (of high copy plasmids/bacteria), quantity check of incoming DNA samples, design and synthesis of primers, manual editing and assembly of all sequence data and a complete report.
The client is regularly informed of the projects progress. The report in PDF format includes the assembly and the resulting contig sequence in FASTA format. On the clients request the report may also be sent by regular mail.
The service includes:
- purification of DNA samples
- Construction of a shotgun library if necessary
- Primer design and synthesis
- Assembly and editing of sequence data
- Comparison to reference sequence if requested
- Full report
BC-160 - PROJECT short2 sequencing reactions with forward and reverse primers on construct < 1200 bp. Data assembled with reference sequence and edited. |
BC-103 - PROJECT singlestrandedComplete sequencing of a construct, including DNA purification, primer design and synthesis, data assembly, editting and comparison with reference sequence. Minimum of one run per basepair |
BC-102 - PROJECT doublestrandedComplete doublestranded sequencing of a construct, including DNA purification, primer design and synthesis, data assembly and editting. Minimum of two runs per basepair (additional coverages possible) |
BC-170 - PROJECT ultrafastComplete sequencing of a construct (<10kb) in 5 days when reference sequence is known. |
© 2005 - BaseClear B.V.
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |