Quick shot

Quick Shot Sequencing service

The Quick Shot sequencing option is our most cost effective product for DNA sequencing. The customer premixes DNA and primer and BaseClear performs a DNA sequencing reaction and analysis of the results.

• For this service the sample and primer are delivered premixed by the customer.
• Only deliver column purified DNA (plasmid or PCR product) mixed with a primer optimised for DNA sequencing
• Sequence reactions are loaded on our sequencers with a run-time corresponding to 800 bases.
• All sequence results, including a report, are sent directly to the customer without any editing or quality check.
• The filenames of the results are renamed, starting with the filename of the reaction from our database and subsequently the sample name taken from the order form.

Only deliver column purified DNA in 1.5ml Eppendorf tubes, dissolved in ultra-pure water or 10mM Tris (pH8.0). Please note that DNA samples should be free of EDTA and ethanol since trace amounts of these compounds will inhibit the sequence reaction.

For purified plasmid DNA submit 0.75 µg plasmid DNA with 25pmol of primer in a total volume of 20µl.

For PCR DNA submit 15 ng per 100bp purified PCR product with 25 pmol of primer in a total volume of 20µl.

E.g. for a PCR-product with a size of 800bp send 20µl sample containing 120ng PCR-product and 25pmol primer.