Special Sequencing
| Richard de Winter |
|---|
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Call: +31 (0)71 523 3917 |
| Contact us |
| Order now |
| Specially for difficult samples |
| Including manual editing |
| Repetition of failed reactions |
| Read length depended on sample/template |
| Delivery time: 3 working days |
BC-180 - GC-rich runAdditional charge for GC-rich samples. Reaction is performed with modified sequencing kit |
BC-500 - Sequencing Primer design and synthesisDesign of a sequencing primer, including synthesis of one primer on 40 nM scale |
BC-201 - Plasmid miniprepPurification of plasmid DNA from 5 ml. of E.coli culture. |
BC-202 - PCR purificationPurification of PCR products prior to downstream procedures. |
Special Sequencing
As a specialist in DNA sequencing, BaseClear has developed special efficient protocols for sequencing samples that cannot be sequenced easily by straightforward normal sequencing.
Services available for large DNAs (genomic DNA, BACs), siRNA templates and sequencing of oligonucleotides. Sequencing includes manual editing if necessary, checking DNA-concentration of samples and repetition of failed reactions.
The services can be complemented with DNA purification and other services.
BC-120 - LARGE construct runsingle sequencing reaction on large constructs (BAC, PAC, cosmid or lambda DNA), including DNA quality check editing of data and rerun if necessary. Readlength up to 400 bases |
BC-130 - GENOME runsingle sequencing reaction on genomic DNA (< 5 Mb), including DNA quality check editing of data and rerun if necessary. Readlength variable. |
BC-150 - siRNA sequencingsequencing of siRNA constructs using runs from both sides of the siRNA and using 2 (different) sequencing kits |
BC-180 - GC-rich runAdditional charge for GC-rich samples. Reaction is performed with modified sequencing kit |
BC-140 - oligo sequencingVerification of an oligo DNA fragment by DNA sequencing |

