PCR Cloning
| Erwin van Rijn |
|---|
 |
Call: +31 (0)71 523 3917 |
| Contact us |
| Order now |
Cloning of a PCR fragment in a vector (standard or client vector) |
Check of >12 clones via colony PCR |
Check of 5 positive clones via double strand sequencing |
Delivery time: 10 working days (standard vector) |
BC-213 - Mutagenesis pointIntroduction of a pointmutation in a DNA fragment cloned in a plasmid vector |
BC-220 - Mutagenesis deletionIntroduction of a deletion in a DNA fragment cloned in a plasmid vector |
BC-103 - PROJECT singlestrandedComplete sequencing of a construct, including DNA purification, primer design and synthesis, data assembly, editting and comparison with reference sequence. Minimum of one run per basepair |
BC-102 - PROJECT doublestrandedComplete doublestranded sequencing of a construct, including DNA purification, primer design and synthesis, data assembly and editting. Minimum of two runs per basepair (additional coverages possible) |
PCR Cloning
Recombinant DNA cloning is the basis of most DNA research projects. The specialists of BaseClear can assist in virtually any step in cloning of DNA. This service can be used to clone a PCR product (up to 2000 base pairs) in a standard vector or client specific vector (provided by client). The sequence of the PCR product will be verified by colony PCR and sequencing 500 base pairs on both sides of insertion point with standard primers located near the insertion point of the fragment.
BC-660 - PCR cloning, 3 checkCloning of a PCR fragment in a plasmid vector, including DNA sequencing of 3 clones |
BC-662 - PCR cloning, 5 checkCloning of a PCR fragment in a plasmid vector, including DNA sequencing of 5 clones |
BC-661 - PCR cloning, additionalDNA isolation and sequencing of additional clones, per clone |
BC-663 - PCR A-tailingAdding of an additional A-nucleotide prior to cloning into a plasmid vector |
© 2005 - BaseClear B.V.
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |
BaseClear | Einsteinweg 5 |
+31 (0)71 523 3917 |