Primer Design*

Oligonucleotide design and synthesis*

Hairpin loops, (cross-) dimers and false priming are a couple of factors that will determine the efficiency of your oligo in a PCR/sequence reaction. With this service we will design oligonucleotides and avoid such factors for you. Other factor as Tm and GC contents can be adjusted to customers wishes. Provides us with the target sequence and the range in which primer(s) should be design and we will provided you with the best possible primer design. As off 1-9-2005 we will have in house oligosynthesis, after that date no data leaves BaseClear and is confidentiality guaranteed! All oligonucleotide come as desalted freeze-dried, complete with a report. As extra service and for oligonucleotides >30-40 mer, oligonucleotides can be purified with reverse phase chromatography (90% pure full-length oligo).

Digital form of the target DNA and the range in which the primer(s) should be designed.

* This service can only be offered in combination with sequencing orders.