Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of bacterial species. The procedure characterizes isolates of bacterial species using the DNA sequences of internal fragments of multiple (usually seven) housekeeping genes. Approximately 450-500 bp internal fragments of each gene are sequenced and compared to a public database for identification.

The great advantage of MLST is that sequence data are unambiguous and the allelic profiles of isolates can easily be compared to those in a large central database via the internet (in contast to most typing procedures which involve comparing fragment sizes on gels).

A further advantage of MLST is that the allegic profiles of isolates can be obtained from clinical material by PCR amplification of the seven house-keeping loci directly from CSF or blood. Thus isolates can be precisely characterised even when they cannot be cultured from clinical material.

BaseClear can assist in all steps in MLST, including:

• DNA isolation 
• DNA amplification (PCR) of the genes 
• PCR purification 
• DNA sequencing 
• Analysis of the results

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