SITE-SATURATED LIBRARY SERVICE
BaseClear can construct high quality synthetic DNA libraries in order to optimise even the most complicated genes. A typical site-saturated library project includes the following stages:
- Discuss and agree with our product specialist which mutants have to be introduced and the backbone vector which can be used
- Create the mutants required at each amino acid position (usually with a guarantee that at least 16 amino acid variants are produced per position)
- The mutants introduced are verified by DNA sequencing and clones containing each of the mutants are cherry picked into a MTP in a format which has been agreed with the customer
- The customer will receive the sequence verified clones in the format required
You submit …
- Sequence of the gene of interest with the positions of interest indicated
- DNA of the target gene and vector
You receive …
- A DNA library as isolated DNA, colonies on plates or colonies picked and transferred into microtiter plates as glycerol stocks
- Report of the library construction including several control steps
- The exact delivery time will depend on the specifications of your project.
Why BaseClear?
BaseClear offers high quality sequencing results and excellent support from our product specialists. We offer validated methods that satisfy the requirements of regulatory agencies.
