PCR CLONING

BaseClear offers an attractively priced standard PCR-cloning service. For a fixed price, we will execute your PCR cloning project and verify the results with DNA sequencing, all within 10 working days.

Our PCR cloning service in short:

• Send in a minimum of 100 ng of PCR products containing one specific band and include a gel picture OR send in a minimum of 50 uL raw PCR product
• Maximum length of PCR products is 1500 bp
• For efficient cloning, PCR products need to be 3’ adenylated and purified. Please indicate on the sample sheet whether we need to perform these additional steps for you 
• Positive clones containing inserts of the indicated length will be identified by colony PCR, and inserts of 3 clones will be verified by sequencing using vectorprimers 
• When available, the sequences will be aligned to a reference sequence
• BaseClear guarantees the complete confidentiality of all client information, project data and biological samples during or after the termination of the project
• In case of gene toxicity, the client is olbiged to pay the full price. Gene toxicity will be determined by comparing ligation efficiency and transformation efficiency.

Optional possibilities:

• PCR product purification
• A-tailing
• A small aliquot of the isolated clones can be sent back to the customer as purified DNA