FROM GENE TO PROTEIN

Date	1 April 2008
Attachments:	schema proteinservice.jpg

BaseClear offers a complete package of services from gene synthesis to protein purification.

Our experience in this field coupled with our in-house gene synthesis and DNA sequencing services enable us to complete projects in a fast and flexible manner. Regular and open communication with our customers is an essential aspect of all projects we perform.

Gene synthesis
If required genes can be synthesized allowing the possibility to optimize codon usage. This can in turn lead to increased expression levels. See our seperate webpage about gene synthesis. http://www.baseclear.com/labservices/synthesis/gene_synthesis

Cloning into expression vector
Genes will be cloned into an expression vector. As standard the vector will contain a purification tag (HIS or strep). Cloning into the customer’s vector of choice can also be performed allowing the addition of promoters, tags or restriction sites.

Protein Expression
Proteins will be expressed in an E. coli expression strain. The protein expression level will be analyzed by SDS-PAGE or Western Blot. Additionally protein solubility can be determined. Upon request, the protein can be expressed in other hosts. Please send an e-mail to Derek.Butler@baseclear.com if you are interested in cloning and expression of a gene in one of the following hosts: Bacillus, Lactococcus, Insect cells, Yeast, Fungal cells.

BaseClear can analyze and optimize the expression level of the desired protein. This is achieved by analyzing and optimizing a number of different parameters including the choice of host strain, the level of induction and growth conditions.

Protein Purification
Small scale purification can be performed using His-tags to demonstrate both tag and protein functionality. Upon request, the protein purification can be optimized and performed at a scale of 10-20 mg.

If you want to know more about our services From Gene to Protein : http://www.baseclear.com/labservices_contact/custom/protein_expression_and_purification

Results
As a result of the agreed procedure (standard or custom), we will deliver:
- Gene cloned into an expression vector, in an E. coli expression strain.
- Small aliquot of tagged purified protein (provided the expression level is sufficient).
- If protein purification is requested, we can deliver up to 10-20 mg of purified protein.
- Report of the expression analysis and (if performed) the protein purification.