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Joris Sostman
Joris Sostman

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Competent Cells

BaseClear offers chemically competent strains of E.coli having transformation efficiencies >108 transformants/μg pUC19 DNA. A unique method was developed to make E. coli strains chemically competent and to avoid the requirement of heat-shocking and related procedures. This dramatically reduces processing time, and, in most instances the entire procedure is complete in 10-20 minutes bypassing the need for a lengthy outgrow period.
For transformation, simply mix DNA with competent cells, incubate on ice for 10-20 minutes, and spread on a selection plate.