- Project Sequencing
- - All steps / Ultrafast service
- - Standard primer list
- - Download sheet
Jan Peter Heutink
Jan Peter manages a team of five technicians, who are responsible for carrying out all DNA purifications and DNA sequencing. Jan Peter takes care of the quality assurance and the delivery of the resulting data at the agreed upon times. He always runs a final check on the sequence data before they are sent off to the customers.
CALL +031 (0)71 523 3917
MAIL info@baseclear.nl
project sequencing service
BaseClear's project sequencing service (also called primer walking) includes all steps that are necessary to obtain a reliable contiguous sequence from your clones. A convenient option.
BaseClear's project sequencing service includes:
- Purification of DNA samples.
- DNA concentration measurements.
- Design and synthesis of all necessary primers.
- Optimal sequencing reaction, if necessary under specific conditions, e.g. in case of GC-rich DNA.
- Assembly and (manual) editing of the sequence data.
- Comparison to a reference sequence if requested.
- Customer will be informed regularly about the progress.
- Extensive advice for special projects.
- Delivery time depends on read length and sequencing strategy. In case of primer walking we analyze > 1 kb per week. Delivery time for the ultrafast project service: 5 working days.
Single-stranded or double-stranded sequencing
Sequencing can be performed for one or for both DNA strands. Double-stranded DNA sequencing results in a higher accuracy of the sequence data. If a reference sequence is known, BaseClear can design the necessary primers on the basis of this. If a reference sequence is not available, or if a clone contains unknown inserts, the DNA can be sequenced via primer walking. Due to the overlap of the resulting sequences, the complete contiguous DNA sequence can be determined.
Ultrafast service
For constructs < 10 kb it is possible to perform the complete sequencing project within 5 working days. This ultrafast service is only possible if a reference sequence is known.
Options
- PROJECT short: Two sequencing reactions with forward and reverse primers on a construct < 1200 bp. Data will be assembled with a reference sequence and edited.
- PROJECT single-stranded: Complete sequencing of a construct including DNA purification, primer design and synthesis, data assembly, editing and comparison to a reference sequence. Only one strand is sequenced using forward or reverse primers. Each base pair will be sequenced at least once.
- PROJECT double-stranded: Complete double-stranded sequencing of a construct including DNA purification, primer design and synthesis, data assembly, editing and comparison to a reference sequence. Both strands are sequenced using forward and reverse primers. Each base pair will be sequenced at least twice (additional coverage is possible upon request).
- PROJECT ultrafast: Complete single-stranded sequencing of a construct (< 10 kb) in 5 working days. Only possible if the reference sequence is known.
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