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Derek Butler

Derek is responsible for the Genome analysis services, which are based on next generation sequencing technology. Together with Erwin van Rijn he also handles the Gene to Protein services. In addition to this, he works on a number of collaborative research projects with universities and research institutes the objective of which is to develop novel and more efficient DNA-related services.

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Protein expression and purification

   

BaseClear offers a complete range of services from gene synthesis to protein purification.

Our experience in this field coupled with our in-house gene synthesis and DNA sequencing services enables us to complete projects in a fast and flexible manner. Customers can choose for a standard feasibility study on protein expression and purification or a custom service for protein expression optimisation or protein purification.

Feasibility study

In a feasibility study we will clone a gene of interest into a suitable expression vector, grow and induce a host strain and determine the protein expression level. In addition to this, we determine the solubility of the expressed protein. In the event of an affinity tag, a small-scale test will be performed to determine the purification possibilities. The total costs of the feasibility study amount to 750 euro. In case of no expression the price is 600 euro.

Custom service: optimisation of the expression

The expression level of a protein will be optimised in a number of stages, tailored specifically for each specific protein and subject to agreement with the customer before initiation.

  • Codon usage optimisation: The codon usage of the gene will be compared to that of the host strain and if necessary, adapted. See our separate webpage about gene synthesis for more information.
  • Introduction of fusion proteins: The fusion of a gene of interest to genes encoding known solubility enhancing proteins can improve the solubility and expression level of a protein.
  • Host strain optimisation: the expression construct will be introduced into different E. coli strains, specifically designed for protein expression. Please, contact Derek Butler if you are interested in other host strains, such as yeast or bacillus.
  • Induction level optimisation: A range of inducer concentrations and induction periods will be tested to determine the optimal conditions.
  • Growth condition optimisation: A range of growth temperatures and growth media will be tested to determine the optimum.  

Custom service: protein purification

If  good protein expression levels are achieved, we can purify the protein by means of affinity columns. Depending on the expression level, up to 30 mg of protein can be produced. Together with the customer we can optimise the buffer and elution conditions to obtain a protein that has the maximum possible activity.

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+31 (0)71 523 3917info@baseclear.com