Order now!
  • Synthetic library 
  • - Optimize activity of a gene 
  • - Site mutated libraries 
  • - Download sheet

Erwin van Rijn

Together with Derek Butler, Erwin is responsible for BaseClear's Gene to Protein services. He focuses mainly on gene synthesis, synthetic library construction, and cloning and primer synthesis. Erwin manages the team of technicians who carry out the laboratory work and solve any problems that might arise. In addition to this, he maintains the contacts with customers about these services.

CALL +031 (0)71 523 3917
MAIL info@baseclear.nl

synthetic library construction

  

BaseClear can create a synthetic DNA library to optimise a gene sequence with a view to improving enzymatic activity.  

A synthetic DNA library can be used to optimise the activity of a gene. A synthetic gene library is synthesised with variations at one or more positions. The variants are cloned into standard or custom vectors and transformed into suitable host bacteria. Customers can then screen the resulting library with activity assays to find the optimal gene sequence.  

Several options:

  • Site Mutated Libraries: One or more specified mutations at a specific location.
  • Site Saturated libraries: One location mutated in all possible variants.
  • Combinatorial Libraries: Several locations mutated in one or more (or all) possible variants. 

Automatic colony picking

We have fully automated systems for the isolation and quantification of DNA from various sources.

After cloning and transformation into E. coli, the colonies can be picked up by our automatic colony picking equipment and transferred into microtiter plates. This allows for fast automatic screening of the libraries. In combination with our in-house equipment for sequencing and gene synthesis, this results in short time lines and high quality libraries.

More information? Fill in our contact form >>

Related Pages:

 

+31 (0)71 523 3917info@baseclear.com