• Real-time PCR 
• - Accurate copy 
• - New assays 
• - Download sheet

Monika Hidding

Monika works at Baseclear as forensic expert and genotyping specialist.

CALL +031 (0)71 523 3917
MAIL info@baseclear.nl

Real-time Pcr service

Real-Time PCR technology is an ideal technique for very accurate copy number determinations. BaseClear can perform standard real-time PCR assays or develop a customised new assay, including the required set of reference genes.

Accurate copy number determination for many samples

Real-time PCR is a fast and accurate method for determining copy numbers of one or more genes in large numbers of samples. In this method, the amount of amplified DNA is coupled to the measured fluorescence intensity via the presence of a fluorescent reporter molecule. Based on the number of PCR cycles that is needed to pass a certain threshold value, it is possible to calculate the initial amount of RNA or DNA. The technique can also be used for SNP and mutation screening.

Standard real-time PCR assays

BaseClear can perform standard real-time PCR assays, supplied by Applied Biosystems. Our well-equipped laboratory with our automated PCR and sequencing facilities enables us to perform these assays fast and accurately within five working days.

Design of new real-time PCR assays

In addition to this, we are able to design a completely new real-time PCR assay targeting the genes of your choice. Specialised software allows us to design the optimal probe sets for an accurate copy number determination.

Reference genes

In the case of RNA samples, it is necessary to incorporate a set of reference genes in order to estimate the efficiency of the conversion from RNA into cDNA. We have standard sets of reference genes for a variety of species. If necessary, we can design a new set of reference genes that meets your requirements exactly.

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