BaseClear can analyze the complete microbiome that is present in your samples. Usually we do this based on PCR amplification and sequencing of taxonomic (e.g., 16S/18S/23S rRNA genes or ITS) or functional marker genes. We compare the generated data to microbial sequencing databases and analyze the data with our in-house developed tool, the BaseClear metaGenome Browser (BmGB)™. In this way we can identify thousands of microorganisms in one analysis, including non-cultivable ones, and determine their relative abundance.
Instead of analyzing only the specific genes of the microbiome (as in the microbial profiling service), BaseClear BiaNomics also offers the possibility to analyze the complete collective genomes of the microorganisms present in a sample. This is referred to as metagenomics or community genomics. We use a whole genome sequencing protocol for metagenomics, including next-generation sequencing. The short reads are assembled into metagenomes according to an optimized in-house protocol. In addition, we can analyze the total extracted RNA from a sample, showing which genes are predominantly expressed in the present microbiome.
Tom is responsible for all metagenomics and microbial profiling projects, which are based on our next generation sequencing technologies, and the development of related approaches.
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