Benchmarking and improvement of the BaseClear 16S rRNA gene profiling pipeline.
16S rRNA gene based microbial profiling remains an important tool to determine the composition of micro-organisms in a myriad of environmental samples, including faeces, soil, water as well as samples derived from food products. Though the technological methodology by itself is reproducible, the influence of DNA extraction, primer specificity, PCR protocol, sequencing and data analysis may affect the outcome of the microbial profiles. As a result, the profiles may not accurately represent the true bacterial composition in the original sample. Here, DNA extraction and 16S rRNA gene profiling was evaluated by characterization of so-called a mock community consisting of 8 bacterial strains.Read more