Summary Experts Meeting Human Microbiome & Applications in Therapies
On March 19th, 2021 BaseClear has organised an online experts meeting on Human Microbiome & Applications in Therapies. With 100+ attendees, this public event was …Read more
Recently, a seismic shift occurred in the probiotics world when the entire Lactobacillus genus underwent reclassification and renaming. There were several very sensible reasons why this was done. The Lactobacilli had become a behemoth: the genus was one of the largest among the bacteria and consisted of microorganisms from diverse environments and with differing phenotypes, which caused communication confusion. In addition, new genome sequencing techniques allow a more reliable classification of microbes, and the current taxonomy of Lactobacillus was considered to be highly irregular. Despite these practical advantages, the name change will have negative consequences for regulatory bodies, industry and the general public as we have to get used to the new names and the microorganisms that they represent.
The Lactobacillus genus was first described in 1901 by renowned Dutch microbiologist Beijerinck. By March 2020, the genus had grown to include 261 species. But how does that compare to other genera? In an analysis of publicly available bacterial genomes, 3525 species had been identified representing 853 genera. On average, each genus only contained 4 species. The genus with the largest number of species was Lactobacillus, or 5% of all genomes were classified. And the number of species in Lactobacillus was steadily rising each year as new species were discovered.
Lactobacillus is an important genus for human health since many types are found in the human microbiome. Lactobacilli are commercially important because they are used as starter cultures for various foods including yoghurt, cheese, sauerkraut and sourdough bread. Many probiotics contain various Lactobacillus species. An important part of the production of silage for animal feed is the reduction in pH by Lactobacillus. Some Lactobacillus species are spoilage microbes for beer and wine.
The reason for the large number of Lactobacillus species was due to its broad definition. The genus comprises bacteria that are rod-shaped and able to produce lactic acid from sugars. Based on these fairly open criteria, a wide range of different bacteria could be placed in the genus. Over the years, it had acquired a wide range of different microorganisms from diverse backgrounds that shared a commonality in form and metabolic activity. However, modern genomic analysis has given us further insights into how closely microorganisms are related. Accordingly, the genetic diversity in Lactobacillus was also found to be much higher than in other bacterial genera, or even families. A further consideration was the normal habitat of the Lactobacillus – some were adapted to the microbiome of certain animals and it made sense to have them grouped together. Microbiologists found that this diversity meant it was difficult to discuss the genus in its entirety.
The move to reclassify Lactobacillus started in 2018 when Salvetti and co-workers published a comparative genomics analysis showing that the entire genus could be subdivided into 10 new genera that reflect how closely individual species are related to one another. However, there were concerns that this reclassification would not be far-reaching enough given the economic, medical and scientific importance of Lactobacillus, and that another reclassification would be needed in the not-so-distant future. An international group of scientists convened to make additional recommendations regarding the most practical approach to be taken.
After careful consideration, the entire Lactobacillus genus was divided into 25 genera. The species part of each bacterial name was retained. Both Lactobacillus and related genus Paralactobacillus were retained, albeit with an adjusted genus description to match the new groupings. 23 new genera were given names, many of which begin with “L” so that prior scientific work could be easily understood within the new system. In addition, the genus Leuconostoc has been added to the Lactobacillaceae family. The Lactobacillus genus was retained for its “signature” species Lactobacillus delbruckii and other similar microbes such as Lactobacillus acidophilus. For the full details, see the website http://lactobacillus.ualberta.ca/, which allows you to search for the old Lactobacillus species and find its new name.
The name changes are expected to have an impact across academia, industry, regulatory institutions, and medicine. Even consumers may be affected. While the new taxonomy does not alter the microbes’ characteristics, meaning current safety assessments are still valid, changing names requires many changes in documentation, databases and other tools that use Lactobacillus species names. Documentation including strain databases, production documents, product dossiers and product packaging will all need to be revised. For example, the probiotic bacterium formerly known as Lactobacillus casei has been renamed to Lacticaseibacillus casei – a small change, but one that could be puzzling for the end-user, and also requires more space to print on a label.
“The Lactobacillus name change project will have far-reaching consequences, both positive and negative, for everyone working with these important microbes,” says Adalberto Costessi, Product Manager Genomics & Regulatory Affairs. “In the short term, a pragmatic approach is needed from all parties involved.”
The European Food Safety Authority (EFSA) has issued a guidance document for affected species in its Qualified Presumption of Safety list. Microorganisms on this list are presumed to be safe after a thorough review has been performed. Before the taxonomic changes, 37 Lactobacillus on the list were reclassified. Information about each species will be transferred to the new name and both names will be retained. Likewise, the Food and Drug Administration (FDA) will take a lenient approach towards potential regulatory hassles from the name change, according to Bartos and Wong.
For scientists, the updated taxonomy will help future research efforts as similar microorganisms are now grouped together in a more logical way. However, prior research that looked at the Lactobacillus genus as a whole will be difficult to interpret now. For example, how can human microbiome studies that found a change in Lactobacillus species due to a certain intervention be used going forward? Clearly, it will take a few years to transition to the new nomenclature.
The characterisation of Lactobacillus-like microorganisms is now more meaningful, as it is clustered along shared genotypic and phenotypic characteristics. Once we get used to it, the new taxonomy will improve our understanding of Lactobacillus and the communities that they exist in.
Register now for our next online experts meeting on “Discovery of Novel Microbial Strains and the Impact of the Nagoya protocol“ on November 24th, 2020. In this meeting experts from the Centre for Genetic Resources, DSM Nutritional Products, WUR, SINTEF and BaseClear will share their knowledge and insights on how the Nagoya protocol influences the discovery and industrial use of microbial strains. In addition the reclassification of the genus Lactobacillus is discussed, including the impact this has on commercial use and regulatory aspects of these strains.
Barco RA, Garrity GA, Scott JJ, Amend JP, Nealson KH, EmersonD. A Genus Definition for Bacteria and Archaea Based on a Standard Genome Relatedness Index. mBio Jan 2020, 11 (1) e02475-19; 10.1128/mBio.02475-19
Bartos J, Wong A. Probiotic taxonomy change has widespread industry impact. July 20, 2020. https://www.nutritionaloutlook.com/view/probiotic-taxonomy-change-has-widespread-industry-impact
Salvetti E, Harris HMB, Felis GE, O’Toole PW. Comparative Genomics of the Genus Lactobacillus Reveals Robust Phylogroups That Provide the Basis for Reclassification. Applied and Environmental Microbiology Aug 2018, 84 (17) e00993-18; DOI: 10.1128/AEM.00993-18
Zheng J, Wittouck S, Salvetti E, Franz CMAP, Harris HMB, Mattarelli P, O’Toole PW, Pot B, Vandamme P, Walter J, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. International Journal of Systematic and Evolutionary Microbiology 2020;70(4):2782-858. https://doi.org/10.1099/ijsem.0.004107.