GENOME DE NOVO SEQUENCING
For genomes that need to be sequenced for the first time, we offer our De Novo Sequencing service. With our many years of experience, highly qualified specialists and state-of-the-art technologies we can assist you with even the most challenging De Novo sequencing projects. We can provide complete start-to-finish project management with customized analysis and data reporting. Our highly qualified staff is always willing to discuss your project to see how we can help you reach your research goals as fast as possible.
For most De Novo sequencing projects we recommend a hybrid strategy. With the SMRT technology (Single Molecule Real Time sequencing) of either the PacBio Sequel or RS II platform we can offer long sequence reads (up to 30 kbp, and average of 3-4 kbp’s / read) to create a backbone. We will also sequence your genome on the Illumina HiSEQ2500 platform (single or paired-end reads) for assembly pollishing to produce high-quality microbial genomes with the lowest number of contigs. Final gaps (if any) can be eventually closed using direct Sanger sequencing and/or long range PCR-based approaches.
EXPERTISE IN MICROBIAL GENOMES
Although we have completed many projects involving human, animal or plant genomes, we have a strong focus on microbial genomes (bacteria, fungi, , yeast, algae, viruses, with genome sizes ranging up to 50 Mbp). We have sequenced and assembled significant numbers of bacteria (including E. coli, Bacillus, Lactobacillus, Lactococcus, Actinomycetes, Neisseria), fungi (such as S. cerevisiae, Aspergillus, Tricoderma) and viruses (Influenza, Adenovirus, etc.).
Fill in our contact form to discuss your microbial genome sequencing project with one of our specialists.
WHY CHOOSE OUR DE NOVO SEQUENCING SERVICE?
- Best sequencing strategy based on a combination of PacBio Sequel or RS II and Illumina HiSEQ sequencing
- Expertise in microbial genomes (sizes ranging up to 50 Mbp)
- Experience with project sizes ranging from single to thousands of genomes
- Multiplex options available to sequence up to 96 samples in a single lane by adding sequence indexes to a library
- Fast and guaranteed delivery times with our premium RAPID services
- Many satisfied customers worldwide
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