Transfection-ready plasmid DNA in minutes with the ZymoPURE products of Zymo Research
Special promotion! Free 20-valve vacuum manifold with the purchase of any 2 ZymoPURE™ kits
Use promotion code ZPUREVAC when placing your order. This special offer is valid for orders placed before June 30st, 2017 to customers from The Netherlands, Belgium and Luxembourg only.
How long does it take you to complete a midi/maxi plasmid prep?
Empower your research with ZymoPURE™ large-scale (Midi/Maxi/Giga) plasmid DNA purification kits. Streamlined methodology and superior technology enables unrivaled speed and performance. Innovative binding technology allows for DNA to be purified using a microcentrifuge column via vacuum or centrifuge in 18 minutes or less.
ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE™ Plasmid kits contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.
Plasmid DNA concentration, yield and transfection efficiency for plasmid DNA isolated using the ZymoPURE™ Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer's suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo™ Luciferase Assay System and Veritas™ Microplate Luminometer. Shown are means + SEM of 8 transfections.
Transfection-ready plasmid DNA in 18 minutes!
- The fastest, easiest, most reliable method for purification of ultra-pure endotoxin-free plasmid DNA
- Plasmid DNA is eluted directly from a microcentrifuge column and is well-suited for transfection and other sensitive applications
- Streamlined workflow:
STILL HAVE YOUR DOUBTS?
Check out this time-lapse video featuring Michiko (one of the Zymo Research researchers) as she completes the process in less than 18 minutes!