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MYCOPLASMA DETECTION

Mycoplasma species are often found as contaminants in cell cultures, virus stocks and other cell-derived chemicals. BaseClear can detect (and identify) these Mycoplasma rapidly and reliably using a validated PCR test. Due to their small size (~0,15 um) and their resistance to common antibiotics such as penicillin and streptomycin, Mycoplasma contamination of cell cultures usually escapes notice. The presence of Mycoplasma however, can cause adverse effects on cells in culture, including changes in growth, morphology, metabolism, protein synthesis and replication.

REQUIREMENT FOR QUALITY CONTROL STANDARDS

Nowadays, testing for Mycoplasma infection is an essential requirement for reaching quality control standards for laboratories concerned with research, production and maintenance of cell lines. Since Mycoplasma contamination is a recurring problem that can cause non-reproducible results in research

and productivity losses in industry, BaseClear recommends routine diagnostic testing of cell cultures on a monthly basis, as well as prior to cell-banking and cryopreservation.

VALIDATED PCR TEST

BaseClear uses a validated PCR test for rapid and reliable detection of Mycoplasma contamination in various in situ biologicals, including cell cultures and virus stocks. The test is based on specific amplification of conserved regions in the 16S ribosomal RNA genes of the Mycoplasma genome. This allows for detection of most of the Mycoplasma, Acholeplasma and Ureaplasma species tested so far. Using this highly sensitive test, we can detect as little as 2-50 Mycoplasma particles of more than 25 different Mycoplasma species, including M. fermentans, M. pirum, M. hominis, M. argininii, M. pneumoniae, Acholeplasma laidlawii and others.

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To maximize test sensitivity, cell lines should be cultured in the absence of antibiotics for 3 days without refreshment of culture media. If cells are cultured for a longer period, inhibiting substances may accumulate in the medium. Collect ~1 ml of cell-free culture supernatant, preferably in screw cap tubes. To maximise test sensitivity, supernatants should be collected from 90-100% confluent cell cultures. Sample tubes should be wrapped in absorbing material and enclosed in a watertight container (e.g. a 50 ml tube) to prevent leaking during transport. The minimum number of samples per order is 5.

You receive…
A full report of the Mycoplasma tests on your samples via e-mail and regular mail, including a table summarizing our interpretation of the test results.

OPTIONAL: MYCOPLASMA IDENTIFICATION

Positive Mycoplasma samples can be further characterised by identification using MicroSEQ®. MicroSEQ® is a revolutionary system for the identification of microbiological samples, based on PCR and sequencing of the 16S/28S rRNA genes. This system provides accurate and reproducible results without the need for time-consuming growth. <Read more >

MYCOPLASMA TESTING DATA 2017

  • 5 and 19 January
  • 2 and 16 February
  • 2, 16 and 30 March
  • 13 April
  • 3, 11 and 25 May
  • 8 and 22 June
  • 6 and 20 July
  • 3, 17 and 31 August
  • 14 and 28 September
  • 12 and 26 October
  • 9 and 23 November
  • 7 and 21 December

Meet Richard de Winter

Richard is director of the Sanger sequencing and microbiology department. In addition he is site-manager of ProBase Pharma, the joint venture of BaseClear and MicroSafe for GMP accredited rapid molecular testing.

Testimonial DuPont R&D

We outsource many molecular biological activities to BaseClear. BaseClear has very good molecular biological equipment and people with extensive knowledge and experience in this field. Even though we have similar capabilities within our own organisation, it makes more sense for us to outsource these activities and apply our focus elsewhere. Important advantage of BaseClear are the quality of their services and their reliability regarding delivery times. Molecular biological work is not always predictable but the staff at BaseClear works very hard to perform conform our wishes and requests on the projects that we outsource to them.

Head Research and Development

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