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ONT Nanopore sequencing update on BioSB Hot Topics meeting

ONT Nanopore sequencing update on BioSB Hot Topics meeting

BaseClear is working on ONT Nanopore sequencing for over one year now, and actively contributes to the relatively large expert-user community within The Netherlands. While Nanopore sequencing technology has tremendous potential, there are still several problems that needs to be solved. Sharing expert knowledge is critical to realize the full potential of this new sequencing era.

For this BioSB organises a Hot Topics meeting on ONT Nanopore sequencing. The goal of this meeting is to bring researchers together and strengthen information sharing and collaboration within the Netherlands and beyond. This day also Adalberto Costessi will give a presentation about the (possible) applications of Nanopore sequencing. The program is aimed at novice and expert users alike.

This meeting will be held on April 20th, 2017 at the TU Delft. Registration is free of charge but required. For more information and registration click here.

The complete program:  

  • 12:15 Lunch and registration
  • 12:40 Welcome
  • 12:45 Introduction -- Thomas Abeel, TU Delft
  • 13:00 Lightweight sequencing of massive genomes -- Christiaan Henkel, Leiden University
    Nanopore technology promises to make long-read sequencing of large eukaryotic genomes both feasible and affordable. As a first example, we have recently assembled the genome of the European eel, using a new bioinformatics approach specifically designed for very large genomes.
  • 13:45 Microbial genomics and metagenomics applications of ONT nanopore sequencing:  Adalberto Costessi, BaseClear
    Single-contig microbial genomes assembled within a day. Composition of metagenomics samples unravelled within hours. Microbial contaminants and viral infections identified within minutes. These are just some examples of groundbreaking analyses made possible by ONT's nanopore technology and that will be discussed during this talk.
  • 14:30 Coffee/Tea Break
  • 15:00 Contributed Show 'n Tell
    Selected workshop participants will briefly present what they are doing with ONT sequencing, what their interests are what their challenges are. You are strongly encouraged to join in.
  • 15:45 Mapping and phasing of structural variations in patient genomes using nanopore sequencing. -- Wigard Kloosterman, UMCU
    We developed a bioinformatic pipeline to efficiently map genomic structural variants (SVs) from the long-read data in human genomes. We demonstrate that nanopore sequencing efficiently captures both complex de novo chromosome rearrangements and common SVs. The long sequencing reads enabled efficient phasing of genetic variations, allowing the construction of genome-wide maps of phased SVs.
  • 16:30 Borrel
  • 17:30 End of the day 


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