Cell line characterization

• 30+ years of expertise in the industry
• Utilizing the latest ONT sequencing technology, with possibility of hybrid WGS approach based on customer needs (combining short-read Illumina and long-read ONT)
• Standardized and customizable downstream analysis pipeline
• Project management supported by in-house scientific experts
• State of the art genomic analysis:
  • Transgene integration site mapping, orientation and copy numbers
  • Detection of: Copy Number Variants (CNVs), Single Nucleotide Polymorphism (SNPs), and Structural Variants (SVs)

Samples

• Human transgenic cell-lines and cell banks
• GMO prokaryotic and eukaryotic production strains
• Microbial cells
• DNA

Deliverables

ONT: Quick and cost effective analysis with long read genome sequencing

• Regular email updates by the BaseClear NGS team about the project status
• Report outlining the procedures performed to obtain the data and data statistics
• Raw sequence data generated by the ONT pipeline and after quality filtering in FASTQ, assembled genome in .fasta file format
• Structural Variants analyses as .vcf file
• Transgene integration site as genomic location.
• Report with brief description of approach and custom findings as .pdf

Illumina: Higher resolution analysis with hybrid genome sequencing

• ILMN sequencing (≥50 x coverage): raw sequence data after quality filtering in FASTQ
• Copy number of transgene as deletion, duplication (it indicates % of success of integration) as .bam and .tsv files
• Single Nucleotide Polymorphism as .vcf file