BaseClear’s Food Safety Digital PCR Panel provides precise, rapid detection of foodborne pathogens, ensuring enhanced safety and compliance for the food industry with reliable, high-quality results.
BaseClear’s Food Safety Digital PCR Panel is a highly versatile diagnostic tool designed to detect and quantify up to seven critical foodborne pathogens. The panel can be customized to meet your specific testing needs, offering multiplex options (up to 7 targets), single-plex assays, or a flexible mix-and-match approach for up to seven targets. The targets included in the panel are:
BaseClear’s Food Safety Digital PCR Panel is ideal for food manufacturers, quality control laboratories, regulatory bodies, and research institutions focused on ensuring food safety and compliance with health standards. This service benefits clients who need to quickly and accurately detect foodborne pathogens in a wide range of food products, including raw materials and processed foods. It is especially valuable for those in the food production, distribution, and testing industries that require high-throughput screening for contamination risks.
The panel addresses key challenges such as the need for rapid, reliable pathogen detection in complex food matrices, compliance with increasingly stringent food safety regulations, and minimizing the risks of outbreaks due to contamination. By providing highly sensitive, multiplex testing, it enables clients to identify multiple pathogens in a single test, reducing time-to-result and increasing operational efficiency.
BaseClear’s Food Safety Digital PCR Panel utilizes the Qiagen QIAcuity One digital PCR platform, a state-of-the-art technology known for its superior sensitivity, accuracy, and reliability in detecting low-abundance DNA targets. The QIAcuity One platform partitions the sample into thousands of individual reactions, enabling precise quantification of pathogen DNA without the need for reference standards or calibration curves. This approach ensures highly reproducible results, even in complex food matrices or samples with low pathogen concentrations.
When using BaseClear’s Food Safety Digital PCR Panel, clients receive a comprehensive report with detailed insights into the number of DNA copies detected for each target pathogen. This report clearly outlines the presence or absence of each pathogen, along with the exact copy number of each target in the sample. The results are presented in an easy-to-read format, including tables and graphs that provide clear visual representation of the data.
We assess whether the test strain can be cultivated under standard MIC conditions. Where necessary, conditions are adapted to ensure valid and reproducible susceptibility testing.
We define the antimicrobial panel and testing strategy based on regulatory requirements and strain type
Your strain is tested using standardized broth microdilution assays under controlled laboratory conditions
Quality control strains and acceptance criteria are applied to ensure data reliability and compliance.
Results are interpreted in line with EFSA guidance, including assessment of intrinsic versus acquired resistance.
MIC testing performed according to CLSI standards
Integrated wet-lab and bioinformatics workflows
Expertise in distinguishing intrinsic versus acquired resistance
Clear, dossier-ready reporting to support regulatory submissions
Digital PCR offers superior sensitivity and quantification compared to traditional PCR by partitioning the sample into thousands of individual reactions, allowing for absolute quantification of viral DNA or RNA. This enables the detection of very low levels of contamination that may be missed by traditional PCR methods, which are typically less sensitive and often rely on relative quantification.
As specified in 9 CFR 113, the following viral contaminants are detected.
Bovine: Bovine virus diarrhea virus, Reovirus, Rabies virus, Bluetongue virus, Bovine adenoviruses, Bovine parvovirus, Bovine respiratory syncytial virus
Porcine: Bovine virus diarrhea virus, Reovirus, Rabies virus, Porcine adenovirus, Porcine parvovirus, transmissible gastroenteritis virus, Porcine hemagglutinating encephalitis virus.
This panel is specifically designed to test bovine and porcine cell lines, including primary cells, cell cultures, and stocks used in vaccine production or other biopharmaceutical applications. It can also be applied to cell harvests and production batches to ensure the absence of viral contamination before further processing.
Standard delivery time for this service is 5 business days after receiving the sample(s). Premium delivery can be requested, and is 3 business days after receiving the sample(s).
As specified in 9 CFR 113, caprine, and ovine cells shall be tested similar to Bovine cells, and can therefore also be tested.
Genomic analysis to identify antimicrobial resistance genes and support
Evaluation of biologically active metabolites to assess functional and toxicological risks
Demonstration of absence of viable cells and recombinant DNA in final products for regulatory compliance
Integration of MIC, genomic, and safety data into a submission-ready regulatory dossier
Get expert guidance on MIC testing, interpretation, and regulatory strategy to avoid delays in your submission. Start with a free consultation tailored to your project.
