Mycoplasma species are often found as contaminants in cell cultures, virus stocks and other cell-derived chemicals. Due to their small size (~0,15 um) and their resistance to common antibiotics such as penicillin and streptomycin, Mycoplasma contamination of cell cultures usually escapes the notice. Mycoplasma presence in cell culture may have adverse effects, including changes in growth, morphology, metabolism, protein synthesis and replication. BaseClear offers an all-inclusive mycoplasma detection service to rapidly and reliably detect Mycoplasma using a validated PCR test.

REQUIREMENT FOR QUALITY CONTROL STANDARDS

Nowadays, testing for Mycoplasma infection is an essential requirement for reaching quality control standards for laboratories concerned with research, production and maintenance of cell lines. Since Mycoplasma contamination is a recurring problem that may cause non-reproducible results in research and productivity loss. BaseClear recommends routine diagnostic testing of cell cultures on a monthly basis, as well as prior to cell-banking and cryopreservation.

VALIDATED PCR TEST

BaseClear uses a validated PCR test for rapid and reliable detection of Mycoplasma contamination in various in situ biologicals, including cell cultures and virus stocks. The test is based on specific amplification of conserved regions in the 16S ribosomal RNA genes of the Mycoplasma genome. This allows for detection of most of the Mycoplasma, Acholeplasma and Ureaplasma species tested so far. Using this highly sensitive test, we can detect as little as 2-50 Mycoplasma particles of more than 25 different Mycoplasma species, including M. fermentans, M. pirum, M. hominis, M. argininii, M. pneumoniae, Acholeplasma laidlawii and others.

You submit…

To maximize test sensitivity, cell lines should be cultured in the absence of antibiotics for 3 days without refreshing the culture media. If cells are cultured for a longer period, inhibiting substances may accumulate in the medium. Collect ~1 ml of cell-free culture supernatant, preferably in screw cap tubes. To maximise test sensitivity, supernatants should be collected from 90-100% confluent cell cultures. Sample tubes should be wrapped in absorbing material and enclosed in a watertight container (e.g. a 50 ml tube) to prevent leaking during transport. Please note that the minimum number of samples per order is 5.

You receive…

A full report of the Mycoplasma tests on your samples, including a table summarizing our interpretation of the test results.

OPTIONAL: MYCOPLASMA IDENTIFICATION

Positive Mycoplasma samples can be further characterised by identification using MicroSEQ®. MicroSEQ® is a revolutionary system for the identification of microbiological samples, based on PCR and sequencing of the 16S/28S rDNA genes. This system provides accurate and reproducible results without the need for time-consuming growth.

Read more about our bacterial identification service

MYCOPLASMA TESTING DATA 2018

  • January 11, 25
  • February 8, 22
  • March 8, 22
  • April 5, 19
  • May 3, 17, 31
  • June 14, 28
  • July 12, 26
  • August 9, 23
  • September 6, 20
  • October 4, 18
  • November 1, 15, 29
  • December 13, 27

Testimonial DuPont R&D

We outsource many molecular biological activities to BaseClear. BaseClear has very good molecular biological equipment and people with extensive knowledge and experience in this field. Even though we have similar capabilities within our own organisation, it makes more sense for us to outsource these activities and apply our focus elsewhere. Important advantage of BaseClear are the quality of their services and their reliability regarding delivery times. Molecular biological work is not always predictable but the staff at BaseClear works very hard to perform conform our wishes and requests on the projects that we outsource to them.

Meet Ali Vahidnia!

Ali is manager of our Microbiology department, which mainly involves bacterial and fungal identification, microbial typing and virus re-sequencing and discovery projects. Ali performs tasks for quality assurance and the timely delivery of results. He always runs a final check on the data before they are sent off to the customer.

Contact Ali

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