While metagenome analysis provides a wealth of knowledge with respect to the functional potential of the sampled microbial community, it does not provide information about which of these functions are expressed. The latter can be achieved by metatranscriptomics and starts with the extraction of RNA. As the extracted RNA predominantly consists of non-coding RNAs (e.g. ribosomal RNA) that do not give information about functional expression, an rRNA depletion step is performed. This is followed by library preparation and paired-end sequencing on the HiSeq Illumina platform. BaseClear offers complete solutions for metatranscriptomic research and applications. Please contact our Product Specialist Metagenomics (Tom van den Bogert) to discuss your project in detail.