With our Transcriptome sequencing (RNA-Seq) service you can discover and profile the entire transcriptome of your species of interest. With no probes or primers to design, our RNA-Seq method can deliver unbiased and unparalleled information about gene expression levels. BaseClear offers strand-specific transcriptome analysis based on sequencing on the Illumina platforms, as well as full-length transcript analysis on the PacBio Sequel. The sequencing strategy is determined in close collaboration between the customer and our Product Managers.

Why choose our RNA-seq service?

  • Years of experience in human, plant, bacterial and fungal transcriptome sequencing projects
  • Special protocols for bacterial and fungal samples available
  • Guaranteed fast delivery times possible with our premium services
  • Strand-specific mRNA sequencing protocols
  • Our specialists can assist you with the experimental design of your RNA-seq projects
  • Standard and custom bioinformatics solutions
  • Sequencing at the lowest cost per base pair
  • Independent service laboratory
  • Many satisfied customers worldwide

Optional bioinformatics

BaseClear offers standard and custom downstream bioinformatics services for RNA-seq projects, including expression analysis, statistical interpretation, isoform detection and functional gene analysis. Read more about these possibilities on our RNA-seq analysis page.

RNA-seq analysis

Small-RNA sequencing service

Our small RNA discovery and analysis service allows the discovery of rare small RNA without previous sequence or secondary structure information in any organism. BaseClear applies the latest randomized adapter protocols by Bioo Scientific that were developed to drastically reduce the bias in small RNA analysis compared to the standard protocols. With this service we can find novel small RNA and analyse the differential expression of all small RNAs in your sample simultaneously.

OPTIONAL BIOINFORMATICS FOR SMALL RNA SEQUENCING

  • Removal of small-RNA adapter sequences
  • Alignment of and annotation of small RNA sequences based on species-specific datasets (among which miRBase, Ensemble)
  • Counting and clustering of annotated small RNA sequences
  • The bioinformatics strategy will be determined in close collaboration between the customer and our bioinformatics specialist
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Meet Erik Hopmans!

Erik is responsible for the Next-Generation sequencing department, which uses Nanopore, Illumina and PacBio sequencers. Erik graduated in Oncology at the VU University of Amsterdam and he’s always had an affinity with new molecular techniques in his early scientific career, such as CGH and expression microarrays and proteomic analyses. During his time at Stanford University he specialized in developing innovative targeted sequencing approaches on the Illumina sequencing.

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