This exclusive and convenient service includes all steps necessary to determine the complete sequence of (large) DNA fragments. Simply submit your samples and a reference sequence and leave the work to BaseClear. We design and synthesize all primers necessary. If a reference sequence is not available, or if a clone contains an unknown insert, we sequence the DNA by using a primer walking strategy. We assemble the sequence data and compare these to the reference sequence (if available).

OUR PROJECT SEQUENCING SERVICE

  • Easy ordering via our online portal https://orders.baseclear.com
  • Single- or double-stranded DNA sequencing, double-stranded sequencing results in a higher accuracy of the sequence data (additional coverage possible on request)
  • Design and synthesis of all necessary PCR and/or sequencing primers included
  • If necessary template preparation: (RT)-PCR and/or purification of DNA samples
  • DNA concentration measurements
  • Optimal sequencing reactions (e.g. in case of GC-rich DNA)
  • Manual editing of the sequence data
  • Assembly into a contig sequence
  • Comparison of contig sequence to a reference sequence (if available)
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GMP SANGER SEQUENCING

BaseClear offers GMP Sanger sequencing services through our daughter company ProBase Pharma. ProBase Pharma offers a highly precise and reliable sequencing service that covers all steps necessary to determine complete double-stranded sequences. It is commonly used for confirmation of the sequence and integrity of a gene or plasmid and prior to cloning or transfection into a cell line or viral vector.

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Meet Leon de Boer!

Leon is manager of our Sanger sequencing department and responsible for the quality assurance and the delivery of data at the agreed upon times. He always runs a final check before data are sent off to the customers.

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