BaseClear offers an attractively priced standard PCR-cloning service. For a fixed price, we will execute your PCR cloning project and verify the results with DNA sequencing, all within 10 working days.

OUR PCR CLONING SERVICE

  • Send in a minimum of 100 ng of PCR products containing one specific band and include a gel picture OR send in a minimum of 50 µL raw PCR product
  • Maximum length of PCR products is 1500 base pairs
  • For efficient cloning, PCR products need to be 3’ adenylated and purified.
  • Please indicate on the sample sheet whether we need to perform these additional steps for you
  • Positive clones containing inserts of the indicated length will be identified by colony PCR, and inserts of 3 clones will be verified by sequencing using vector primers
  • When available, the sequences will be aligned to a reference sequence
  • BaseClear guarantees the complete confidentiality of all client information, project data and biological samples during or after the termination of the project
  • In case of gene toxicity, the client is obliged to pay the full price. Gene toxicity will be determined by comparing ligation efficiency and transformation efficiency.
  • A small aliquot of the isolated clones can be sent back to the customer as purified DNA
Request quotation

Testimonial DSM Biotechnology Centre

BaseClear offers a good performance from a technical perspective. I really appreciate the open way in which we communicate and whenever we need something done urgently, they are always willing to discuss the possibilities and try to help us make deadlines. They offer advice on the best set-up of the project and concentrate on finding solutions, instead of only pointing out potential problems.

Meet Leon de Boer!

Leon is manager of our Sanger sequencing department and responsible for the quality assurance and the delivery of data at the agreed upon times. He always runs a final check before data are sent off to the customers.

Contact Leon

Convinced? Get in touch

Get a quoteMeet baseClearContact form