Whole Plasmid Sequencing

Oxford Nanopore Technologies’ (ONT) long, continuous reads are ideal for sequencing large plasmids and assembling complex structures, showcasing its versatility across various sample types, including low-quality and degraded DNA. While traditional sequencing methods like Sanger sequencing, remains valuable for specific applications requiring high accuracy, whole plasmid sequencing provides a comprehensive and streamlined approach for studying plasmid biology in diverse research contexts

Our ONT whole plasmid sequencing service delivers high quality, full-lenth sequence information for secure characterisation of plasmids. As a certified ONT service provider, we utilise the latest technology to sequence plasmids, with a short turnaround time of just 1 business day*!

By sequencing the entire plasmid at once, you can quickly obtain comprehensive genetic information without the need for fragment assembly, thereby reducing the risk of errors and achieving more reliable results.

• Quick and easy identification of plasmid insert size and orientation
• Verification of entire vector sequence
• Available from one sample, and also by plate
• Resolves repeat regions, dimers and deletions
• Make detection of contaminants or unexpected products possible
• No complicated primer walking and annealing issues

Clinical microbiology: Studying plasmid dynamics within microbial communities to understand the spread of antibiotic resistance genes and other mobile genetic elements to develop better treatment strategies.

Biotechnology: Engineering plasmids serves diverse biotechnological goals, from protein production to gene therapy and various biotechnological applications such as verifying genetic modifications and ensuring GMO safety.

Environmental monitoring: Assessing the impact of anthropogenic activities on microbial populations and their genetic diversity to facilitate the development of eco-friendly waste management strategies.