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Technology

BaseClear has access to the latest technologies for DNA and RNA research and analysis. Our lab is equipped with the latest sequencing technologies including the Illumina HiSEQ2500, Illumina MiSEQ and PacBio RS II, PacBio Sequel and Oxford Nanopore MinION. With our automated lab protocols and optimized internal processes we can offer our customers high quality results with fast and guaranteed delivery times.

  • Illumina HiSEQ2500

    Illumina HiSEQ2500

    The Illumina HiSeq 2500 System is an efficient and powerful high-throughput sequencing system that supports a broad range of applications, including De Novo or re-sequencing projects and transcriptome analyses (RNA-seq).

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  • Illumina MiSEQ

    Illumina MiSEQ

    BaseClear can offer specialized sequencing services on the Illumina MiSeq desktop sequencer. The MiSEQ system employs Illumina sequencing by synthesis technology (SBS), which is the most widely adopted next-generation sequencing technology.

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  • PacBio RS II (Pacific Biosciences)

    PacBio RS II (Pacific Biosciences)

    BaseClear offers unique services using the PacBio RS II system (Pacific Biosciences). With the PacBio RS II BaseClear can offer offer real-time sequencing of single molecules with read lengths up to 30 kbp.

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  • PacBio Sequel system

    PacBio Sequel system

    BaseClear offers high quality services using the latest platform from Pacific Biosciences, the PacBio Sequel system. The data has added value for de-novo assembly, targeted sequencing, detection of structural variation and the direct detection of base modification. The main advantage of the Sequel system over the PacBio RS II platform is the higher output.

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  • Nanopore MinION

    Nanopore MinION

    Since 2015 BaseClear is working with the Oxford Nanopore MinION sequencer for R&D projects. The MinION is the first portable, real time device for DNA and RNA sequencing. With this sequencer ultra-long read lengths of hundreds of kb are possible.

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  • ABI3730(XL)

    ABI3730(XL)

    BaseClear has over 20 years of experience in offering Sanger sequencing. We have several ABI3730XL and ABI3730 sequencing platforms, which are used for our Sanger sequencing, but we also have dedicated machines for fragment analysis.

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  • Vitek® MS from BioMerieux (MALDI-TOF)

    Vitek® MS from BioMerieux (MALDI-TOF)

    MALDI-TOF identification using the VITEK® MS from bioMérieux is achieved by obtaining spectra using MALDI-TOF technology (Matrix Assisted Laser Desorption Ionization Time-of-Flight) and analyzing the spectra with the VITEK® MS database. The peaks from these spectra are compared to the characteristic pattern for a species, genus, of family of micro-organism, thus resulting in an identification.

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  • MicroSEQ® system: Validated microbial identifications

    MicroSEQ® system: Validated microbial identifications

    BaseClear offers high quality microbial identifications using the validated MicroSEQ® system from Life Technologies. Identification of bacterial and fungal samples by PCR and DNA sequencing using MicroSEQ® validated protocols, kits and database.

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  • Custom sequence identifications

    Custom sequence identifications

    Identification of bacterial or fungal samples by PCR and DNA sequencing of customer requested genes. Primers bind to the conserved regions within the genes, while the divergent regions are sequenced for identification.

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  • Microbial Typing using REP-PCR

    Microbial Typing using REP-PCR

    REP-PCR typing generates a DNA fingerprint that is unique to the organism at the strain level. With a comprehensive, dynamic picture of any microbial environment, technicians can track and manage data at the strain level, letting them pinpoint contamination with a high level of speed, ease and precision.

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  • Multilocus Sequence Typing (MLST)

    Multilocus Sequence Typing (MLST)

    MLST is a technique in molecular biology for the typing of bacterial species. The procedure characterizes isolates of bacterial species using the DNA sequences of internal fragments of multiple housekeeping genes and comparison to a public database for identification.

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  • Genotyping services

    Genotyping services

    BaseClear offers a complete range of reliable, high quality genotyping techniques and services. BaseClear not only performs standard genotyping assays, but can also carry out an entire genotyping project, including the design of a new assay that corresponds exactly to your needs.

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