Zymo Research has developed a range of premade, chemically competent E. coli strains for high efficiency transformation (> 108 transformants/µg pUC19 DNA). These innovative Mix & Go Competent Cells feature a streamlined transformation procedure that eliminates heat shock, long incubations, and the need for electroporation. With the chemically competent Mix & Go E. coli from Zymo Research, a scientist can transform cells in less than 20 seconds… Simply mix and plate!

Zymo Research also provides the reagents so researchers can make their own Mix & Go Competent E. coli from their favourite lab strains. Included is a specially formulated growth medium, ZymoBroth™, which enhances the transformation efficiencies of many K- and B-strains of E. coli. Increase transformation efficiency and decrease transformation time with the Mix & Go system!

For more details about these products, please find our product catalogue at the bottom of this page.

Chemically Competent Cells

The Mix & Go Competent Cells from Zymo Research offer the simplest and most efficient means of E. coli transformation. Zymo Research’s scientists have developed a unique method that completely eliminates the need for heat shock, lengthy incubations, and outgrowth steps. Simply mix DNA with the cells then spread directly to a plate… Mix & Go! The competent cells are highly efficient and can be used for cloning, sub-cloning, library construction, etc.

Check out this video that demonstrates how to use Mix & Go Competent Cells in just 20 seconds!

Transformation Kits & Accessories

Zymo Research has developed a streamlined method for generating Mix & Go Competent Cells from existing E. coli strains. This innovative process eliminates the need for heat shock and related steps typically involved in bacterial transformation. By simply adding DNA to Mix & Go Competent Cells and plating, transformation can be achieved efficiently, with typical efficiencies ranging from 10^8 to 10^9 transformants/µg plasmid DNA across most E. coli strains. The process involves growing the desired E. coli strain, washing, and resuspending the cells in provided buffers.

Additionally, Zymo Research offers ZymoBroth™, a specially formulated growth medium that significantly enhances transformation efficiency compared to traditional SOB medium, with increases typically ranging from 5 to 100 fold depending on the E. coli strain.

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