DNA ISOLATION AND PURIFICATION KITS
Zymo Research DNA purification kits are based on affinity spin columns (as single columns or 96-well filtration plates), containing a unique silica-based matrix. The material offers several advantages compared to standard nucleic acid purification columns:
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- Low elution volumes (6-8 ul)
- Fast and simple protocols (e.g. PCR clean-up within 2 minutes)
- High yields of extremely pure DNA
- No wash residue carryover
- No retention volume, therefore no loss of DNA
- All components can be ordered individually
- Kits are available for the isolation of fragment DNA, plasmid DNA and genomic DNA from many different sources
DNA Fragment Clean-up & Concentration
A variety of DNA kits are available for the rapid desalting, purification, and concentration of DNA from in vitro reaction mixtures and cell-free lysates or from TAE/TBE buffered agarose gels. The DNA Clean & Concentrator kits facilitates the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, restriction endonucleases, etc., as well as free dNTPs and radiolabled and fluorescent derivatives. DNA recovered and concentrated using the DCC™ is ideal for use in subsequent sequencing, cloning, ligation, microarray, and endonuclease digestion procedures. Likewise, DNA recovered from agarose gel slices by the Zymoclean™ product line enables the size selection of DNA for subsequent use in sequencing, cloning, PCR, and DNA labeling reactions
Plasmid DNA Purification
The Zyppy™ plasmid DNA purification kits allow the user to separate plasmid DNA efficiently from chromosomal DNA and cellular RNA in bacterial host cell lysates using procedures that are fast, user-friendly, and reliable when compared to those offered by other suppliers. All of the Zyppy™ kits include color-coded reagents that allow for easy visualization and assessment of bacterial cell lysis and buffer neutralization steps. Also available is the Zymoprep™ line of miniprep kits that allow the user to isolate plasmid DNA from yeast.
Genomic DNA Isolation
Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh and paraffin-embedded tissue sections, cultured cells, saliva, buccal cells, whole blood, plasma, serum, urine, bacteria, fungi, yeast, algae, viruses, and mitochondria. The genomic DNA isolation kits yield high quality dsDNA that is ideal for use in downstream applications such as PCR, Southern blotting, endonuclease digestion, and methylation detection.
Environmental DNA Purification
Bead bashing is often required for the efficient processing of tough-to-lyse organisms and environmental samples. The environmental purification kits feature unique BashingBead™ technology, which allows isolation of DNA from samples refractory to conventional lysis procedures including tough-to-lyse tissues, soil samples, feces, plants, seeds, food, arthropods, Gram positive and Gram negative bacteria, yeast, filamentous fungi, unicellular and filamentous algae, and protozoa. These products lead to high yield and high quality DNA suitable for downstream applications such as PCR, sequencing, hybridization, restriction digestion, and other enzymatic processes.
For the complete overview of products, please visit www.zymoresearch.com.
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