LONG-READ ASSEMBLY STRATEGY FOR DE NOVO SEQUENCING PROJECTS
In order to reach the highest quality genome sequence for De Novo projects, we usually recommend a combination of long (PacBio or Nanopore) and short (Illumina) sequencing technologies which are assembled using state-of-the-art computation algorithms. The long sequence reads comprise the information to generate a proper draft genome architecture, whereas the high-quality Illumina short reads can be used for polishing in order to generate a final assembly of highest quality at both a structural and sequence level. Final gaps (if needed) can be closed using for instance Sanger sequencing.
Advanced pipeline for high throughput genome analysis projects
We have developed a high throughput bacterial sequencing platform which is capable of sequencing literally hundreds of genomes per week. This platform is a combination of in-house developed library preparation solutions, robotics, genome sequencing and several automated bioinformatic pipelines. We can tailor this bacterial sequencing pipeline to your research question and think with you to minimize the cost of sequencing while maximizing the output. Additional options like an annotation of the whole strain collection and view the genomes in a browser will allow you to search for specific genes or enzymes.