Variant detection is an important application of next-generation sequencing experiments. It involves the alignment of sequence reads to a reference and the detection of mutations and other minor variations. While this process may sound simple, in practice many steps and …
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While the European (EFSA) and American (FDA) regulatory bodies have established guidelines for the approval of novel microbial strains in food and feed applications, these guidelines are often changed and in some cases are difficult to interpret, making it challenging …
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Obtaining regulatory approval to use microorganisms as food additive or production strain can be challenging. Applying the regulatory guidelines and rules to the genomic data generated when analysing these microbial strains often presents both technical and interpretation challenges. This article …
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The rapid advancement in the genomics field opened new possibilities in biomarker and vaccine development. The availability of genomic data of pathogens increases the opportunities for the identification of novel vaccine candidates and allows one to move from a conventional …
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Nature contains a wealth of genes, enzymes, metabolites and other biomolecules that might be interesting for new applications. They might be useful, for example, as food ingredients, as enzymes in detergents, as drugs or antibiotics or as biofuel. Such unknown …
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Next-Generation sequencing technology is becoming indispensable for many food manufacturers and labs, as it helps them perform safer, faster and more affordable food safety and quality control programs. However, there are still many food companies that are not familiar with …
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A manufacturer of bottled juices for consumption noticed debris in juice bottles as well as a sour, bad odor. Attempts to obtain microbial isolates from the juices for root cause analysis failed. The manufacturer contacted BaseClear to identify the microorganisms that may …
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A Salmonella detection at several production sites in different countries forced a large multinational to perform a root cause analysis. Fast results were essential to limit damages and preventing an outbreak. BaseClear’s strain typing team reported results within 48 hours, minimizing the financial consequences …
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Within BaseClear we are working with Nanopore sequencing since 2014. And in 2017 we became the first certified service provider from Oxford Nanopore Technologies worldwide. Although we are working with this innovative and ground breaking technology for several years now, …
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In my role as account manager at BaseClear I encounter many scientists and R&D managers that cannot see the wood for the trees when it comes to choosing a suitable sequencing platform. For this reason, I wanted to provide a …
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16S rRNA gene based microbial profiling remains an important tool to determine the composition of micro-organisms in a myriad of environmental samples, including faeces, soil, water as well as samples derived from food products (e.g. milk, yogurt, and beer). Though …
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As the costs for sequencing have significantly decreased over the last years the number of whole genome sequencing (WGS) is vastly increasing. Often the end result consists of assembled and annotated genomes. Researchers are required to submit these to the …
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