- Genome Sequencing
- - High quality
- - Full Service
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Derek Butler
Derek is responsible for the Genome analysis services, which are based on next generation sequencing technology. Together with Erwin van Rijn he also handles the Gene to Protein services. In addition to this, he works on a number of collaborative research projects with universities and research institutes the objective of which is to develop novel and more efficient DNA-related services.
CALL +031 (0)71 523 3917
MAIL info@baseclear.nl
De novo Genome sequencing
For a genome that is to be sequenced for the first time, we offer our De Novo Sequencing Service, based on the paired-end technology.
Approach
Our product specialist works out which combinations of single read, paired-end or mate-pair sequencing have to be performed. This is done in close collaboration with the customer.
Starting material
Genomic DNA (5-10 ug)
Sequencing
- Sequencing with the Illumina GAII
- Gap closure possibilities, based on a combination of direct Sanger sequencing, Next Generation Sequencing and long range PCR-based approaches.
Bioinformatics
- Supply of the raw sequencing data (fasta or scarf)
- Optional: De novo assembly in which we provide the customer with the contigs which are generated
- Optional: Downstream options such as annotation, genome screening and genome analysis.
- The bioinformatics strategy will be determined in close collaboration between the customer, our bioinformatics specialist and Genome Analysis product specialist.
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